Abstract: Abstract: Rude fat and glucosinolates contents in Brissica Napus L. selfing seeds and open pollinating seeds of 861 Brassica nanpus L. materials in Sichuan ecological region were tested by near-infrared spectroscopy ( NIRS ) on a near-infrared grain analyzer, and the test values of two type seeds were compared. The rude fat test values of open pollinating seeds was higher than the values of selfing seeds at large and the difference in results between two type seeds was at 1 percent significant level. Meanwhile, the difference in results between selfing seeds and open pollinating seeds was not significant when glucosinolates contents were concerned. It indicated that the bags using for self pollinating do some influence on the rude fat content in the rapeseeds, so the test values of open pollinating seeds by near-infrared spectroscopy should be pay more attention than the ones of selfing seeds, and the glucosinolates contents test values of selfing seeds and open pollinating seeds have same importance in Brissica Napus breeding selection. The correlation coefficients of rude fat and glucosinolates contents test values of the two type seeds were at 1 percent significant level; The relationships of rude fat and glucosinolates contents test values could be described with linear regressive formulas respectively.