Abstract: Boehmeria nivea (Linn.) Gaud. cultivar “Zhongzhu No1” has been used, 4CL-1 genomic DNA sequence has been cloned first time by PCR with degenerate primers, the length of the sequence is 983 bp; 4CL-1 cDNA sequence has been cloned first time by RT-PCR with special primers from ramie stem, the length of the sequence is 110 bp; Bioinformatics analysis shows that this sequence encode a polypeptide about 37 amino acid residue, this polypeptide is similar to acyl-CoA synthetases, and related to AMP forming and binding.