Abstract: 【OBJECTIVE】Establishment of a rapid, accurate and sensitive one-step IC-RT-PCR method to detect Bean pod mottle virus (BPMV) in soybean seed.【METHOD】One-step IC-RT-PCR was developed to detect BPMV by using RT-PCR in combination with immunocapture. After optimization of the reaction conditions, One-step IC-RT-PCR procedure was established, and then the specificity and practical effect of detection were validated.【RESULTS】The One-step IC-RT-PCR could successfully amplify target fragment from BPMV-infected soybean seeds while not from other virus infected and healthy soybean seeds. BPMV in soybean from different producing areas was detected by means of the One-step IC-RT-PCR. The results showed that BPMV was detected in soybean seed from America and the incidence was 50%.【CONCLUSION】The One-step IC-RT-PCR was rapid and accurate for detection of BPMV in soybean seed, which can be applied to the field of port inspection and quarantine.