Abstract: Pollen development is a very complex process in which many correlative genes play important roles. The study of gene engineering on pollen development is a central linkage between molecular study on pollen development and developmental regulation research in plants. It is helpful for us to understand thoroughly the mechanism of pollen development in plants. The research advances on gene isolation, expression and regulation of pollen development were summarized. Several methods of creating male sterility through gene engineering were discussed. The paper also brought forward application prospects in future.