Abstract: In Vitro culture and micropropagation for different selections of cherry plant hybrid including F8 and F10 (P. avium L.× P. pseudocerasus L.), H8 and H10 (P. cerasus L × P. pseudocerasus L.) were studied in this paper. It was showed that by taking MS and F14 basic medium supplemented with different combinations of plant growth regulators such as 6-BA, IBA and GA3, the micropropagation technologies including preliminary culture, subculture and rooting culture were successfully established. Medium F14+6-BA (0.3～0.5)mg/L+GA30.1mg/L is suitable for preliminary culture of the tested selections, F14+6-BA (0.5-1.0) mg/L +IBA0.1 mg/L +GA30.1 mg/L is an optimal subculture medium for proliferation of the In Vitro shoots, satisfactory rooting response can be obtained on medium 1/2MS+IBA0.5 ( or NAA 0.8) mg/L.