Abstract: During 2006-2007,a field experiment was employed to study on plant growth regulator on chemical compound of different genotypes flue-cured tobacco leaves by using tobacco (Nicotiana tobacum L.) cultivars yunyan87 and jiyan NO.9 in Jilin. Results showed that the content of chemical compound effected differently by distinct genotypes of sprayed plant growth regulators. Yunyan87 is better than jiyan NO.9,and sprayed yunyan87 with the plant growth regulators on the topping day has better harmony chemical compound, and is good for initial quality of flue-cured tobacco.