Abstract: 206 cultivation strains of Agaricus bisporus were studied by using the techniques of SRAP, ISSR and RAPD fingerprinting, and 15 special SRAP bands, 3 special ISSR bands and 2 special RAPD bands were obtained. The strains were clustered based on the 20 special bands and a tree map was obtained. The results showed that 206 strains be divided into high-production group and good-quality group at the similarity coefficient of 28%, while at the similarity coefficient of 100%, the strains were divided into 72 groups with strain number range of 1~31.