Abstract: The research showed that harvest time had the significant effect on oil content and yield of high-oil soybean. With the delaying of the harvest time, the content of oil increased the maximum value at first, and then dropped. The ranges of oil content of the six varieties varied from 0.37 to 0.87 percentage point, the oil content variation of Heinong44 reached very significant different level, Hefeng48 and Hefeng40 reached significant different level. The harvest time had the significant effect on the yield. The yield of four late-maturing varieties increased first, then decreased, and reached very significant different level, but the harvest time have little effect on the two early-maturing varieties.