Abstract: In order to obtain the strains of effectively degrading cypermethrin, the bioremediation bacteria were enriched and isolated from cypermethrin contaminated soil. Three predominant bacteria were obtained, and named as LF-1, LF-2 and LF-3. Of these, the LF-1 was selected for further study for biggest potential of cypermethrin degradation. LF-1 was identified as a member of kurthia sp. based on its biochemical characteristics and homology of 16SrDNA sequence (Genbank Accession No. EU677769). The cypermethrin quantity was analyzed accurately by GC with µECD. The results showed 35℃, pH 7 were the optimum degradation conditions, respectively. LF-1 could degrade cypermethrin up to 80.15% in a concentration of 100 mg/L within 8 d, and also degrade fenpropathrin, bifenthrin effectively. The plasmid cured strain could not degrade cypermethrin, which indicated that the degradation gene was probably involved in plasmid. The degradation characters showed the potential to be used in a bioremediation application in agriculture.