Abstract: Experiment was conducted with Shuguang(Prunus persical.Batsch) for studying the applying of Pollen-tube path way in peach.Results showed: (1)24h after pollination in vivo, pollens germinated on the stigma;After 48h ,96h,144h plenty of pollen-tubes respectively reached to the upper,middle,bottom of style.(2) The best time to inoculate was 144h after pollination; The injection method of adding the crosscut on the stigma was not suitable for peach;The rate of fruit setting was better than other concentration when was 0.2 mg/l.