Abstract: Two sweet pepper genotypes were analyzed by 4 kinds of reagents on 9 different levels,including 2 antibiotics and 2 bactericides. The effect of them on anther swelling, contamination, browning and embryo production were detected. The results indicated that 0.5~1.0 ml/L Shan Nong Yi Hao Ⅲ was good at controlling anther contamination and embryo production, which could be used for sweet pepper anther culture.