Abstract: Candida albicans (C. albicans) and other fungi an important human and veterinary pathogens. At present, the application of effective clinical drugs are limited, because the drugs have high toxicity and are easy to have a resistance that are causing the rate of relapse and re-infection rate significantly increased and a number of adverse reactions. There was an urgent need to develop high-performance, low toxicity of new drug against resistant fungi. The minimal inhibitory concentration of thymol (THY) tested against clinical trichophyton rubrum strain BMU 01672, Saccharomyces cerevisiae standard strain and 24 clinical C. albicans, and checkerboard assay was used to analyzed the ractional inhibitory concentration index (FICI) of THY/FLC, THY/AMB. The result showed that THY had good antifungal activity, and THY/FLC and THY/AMB showed synergism in antifungal interaction.