Abstract: Abstract: Objective：To investigate the incidence of two virulence determinants and two phenotypes in 53 strains of enterococci derived Swine. Methods：PCR were used to detect the two virulence determinants in 53 isolates . The two phenotypes , including β-hemolysis and gelatin hydrolyze,were performed on agar plate containing 50mL/L rabbit blood and agar plate supplemented with 30g/L gelatin respectively. Results：In E.faecalis, E.faecium and E.avium, the rates of detection of the two virulence determinants were cylA 94.4%、96.9%、100%, gelE 100%、87.87%、50% , the β -hemolysis was 72.2%、42.4%、0 , the gelatin hydrolyze was 100%、0、0. Conclusions：cylA and gelE are the main virulence determinants in enterococcui derived swine. E. faecalis possess more virulence determinants and phenotypes than E. faecium. E. faecalis derived fresh pork possess stronger virlence determinants indicate we must pay attention to food sanitation. 2 strains E.avium were detected in fresh pork and E.avium possess low virulence by detection of Phenotype and Genotype.