Abstract: Abstract: 2 Pleurotus nebrodensis strains and 2 Pleurotus ferulae strains were used as the materials for the antagonistic test and cultivation test. The results showed that there were great distinguish on morphology and growth characters of the mycelium and food body under the different temperature between them. The days of the mycelium of the Pleurotus nebrodensis strains fully colonized in the PDA slant were 3-5days earlier than the Pleurotus ferulae strains. So as in the cultivate bag. There were obvious antagonistic lines and non-growth region between them. The primordium appeared easily in tube slant medium under the low temperature 15-25℃ of the both mycelium. The color of the fruit body and the time of producing food bodies of the Pleurotus ferulae changed largely with the different sowing time. In autumn the time of producing food bodies were delay with the sowing time delay of the Pleurotus nebrodensis ，but the fruit body morphology had no change. The average biological efficiencies had no significant difference between two strains.