Abstract: 【OBJECTIVE】The reproduction of Rana spinosa David was artificially controlled by using biotechnology, in order to advance and centralize the ovulation, synchronize incubation and batch breeding of tadpole.【METHOD】HCG and LRH-A3 were used to make artificial inducing spawning of R. spinosa【RESULTS】No inducing effect of HCG was found on spawning of R. spinosa. LRH-A3 had obvious effect on spawning, but the insemination of R. spinosa was inadequacy. The combination of HCG and LRH-A3 not only had significant function on spawning, but also made complete insemination. After treated with HCG+LRH-A3 in April to June, the growth time from spawn to young frog was 73-89 d, all before September. 【CONCLUSION】The combination of HCG and LRH-A3 could be used to artificial control R. spinosa in practical breeding.