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Chinese Agricultural Science Bulletin ›› 2008, Vol. 24 ›› Issue (4): 94-98.

Special Issue: 生物技术； 小麦

• 农业生物技术科学 • Previous Articles     Next Articles

Cloning and Sequencing of GAPDH Gene in Common Wheat(Tritucum aestivum L.)

Yue Caifeng, Kang Guozhang, Liu Chao, Guo Tiancai, Zhu Yunji, Shen Bingquan   

  • Received:2008-01-17 Revised:2008-01-23 Online:2008-04-05 Published:2008-04-05

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Abstract: The total RNA of wheat leaves was extracted with Trizol, and the partial cDNA sequence of wheat GAPDH gene(EU022331) was amplified through RT-PCR. The colned GAPDH gene (604 bp) was 95 %, 86 % and 84% identified with to M36650(Barley), AY109397 (maize), BT012693(tomato) , respectively. This partial sequence provided a good background on studying expression of many cereal genes.