Abstract: The content of pectic polysaccharides and other secondary metabolites is high in cell of Okra, which influences frequently the yield and quality of genome DNA when being extracted. In this study, a new method of CTAB was introduced which do best for characterization the purity and concentration of genome DNA. The result showed that it could effectively eliminate the affections of the secondary materials to extracted genome DNA from Okra, and which be suitable for RAPD analysis and other molecular maker.