Abstract: In this paper we test with soil microorganisms from Hevea forest, and direct extract microbial nucleic acid from soil, The research shows that this method of introduction in this experiment not only generate the largest DNA fragments in size, but also it was directly used as PCR amplification and digestion of restriction enzymes and later operation without purification, about 2.1-4.6μg DNA yields could be extracted from one gram soil on average. This method is easy and quickly in operation, and provide the basis of the construction of metagenomic library and diversity analysis of soil microbial communities.