Abstract: the shoot tips of bowl lotus were used as explants for in vitro propagation. The factors affecting the inducibe culture and proliferate mediums and the methods of controlling contamination in culture were studied.The result indicated the suitable timing of taking shoot tips for culture were October to December, the most suitable inducibe medium was 1.0 mg/L 6-BA+0.25 mg/L NAA+1.0 mg/L GA3, the best proliferate medium was MS +4.0 mg/L 6-BA + 0.25 mg/L NAA. the best medium state is liquid-solid state, that is the uper layer’s component is MS + 1.0 mg/L 6-BA + 0.25 mg/L NAA + 1.0 mg/L GA3with 5 mm thickness and the under layer ’s component is MS + 1.0 mg/L 6-BA + 0.25 mg/L NAA + 1.0 mg/L GA3with 1cm thickness.the method of controlling contamination in culture was 75% alcohol 1min and 0.1% HgCl2 10min.