Molecular Marker for the Waxy Protein Subunits in Synthetic Hexaploid Wheat-Derived Populations

Abstract

Abstract: Synthetic hexaploid wheats, obtained by crossing tetraploid wheats (AABB) with Aegilops tauschii Coss (DD), containing genetic variability different from bread wheat, now have been widely used for genetic improvement of bread wheat. A total of 113 BC2F2:6 advanced lines derived from four CIMMYT synthetic hexaploid wheats, Syn768, Syn769 and Syn780, were used to detect Waxy protein by specific-primer PCR amplification and modified PAGE. The results showed that 8 of these cultivars and lines were the null Wx-B1 type and null frequency was 6.6%, and what is more, there was no other null types existing in the materials detected. The null frequency was different between the population from the three parents of synthetic hexaploid wheats. From this study, it could be concluded that the study on Waxy genotype of progeny from crosses of synthetic hexaploid wheat and bread wheat can provide useful information for improving bread wheat and be a useful tools in marker-assisted selection of wheat breeding programme.

Yang Songjie ，Yang Wuyun. Molecular Marker for the Waxy Protein Subunits in Synthetic Hexaploid Wheat-Derived Populations[J]. Chinese Agricultural Science Bulletin, 2008, 24(4): 52-57.

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