Abstract: The genetic diversity and genetic relationships among 34 cherry accessions, including 3 interspecific progenies, 1 Prunus pseudocerasus, and 30 Prunus avium, were evaluated by ISSR markers. In ISSR analysis, a total of 149 bands were detected, among which 143 bands were polymorphic. The number of bands from each ISSR marker ranged from 6 to 12, with an average of 8.76. The PPB, PIC and Ne were 95.97%, 0.90 and 1.914, respectively. The ISSR-derived genetic similarity (ISSR-GS) ranged from 0.44 to 0.91, with a mean of 0.73. The cluster analysis indicated that all the 34 sweet cherry germplasm could be distinguished by ISSR markers. The genetic relationship cherry accessions were analyzed and 34 germplasm were classified on molecular level by clustered analysis, which provided reference for research in cherry germplasm.