Isolation and Characterization of a Rice Gene OsRan1 Coding a Small G Protein

Abstract

Abstract: The disadvantage circumstance is an important factor in impacting on the yield and quality of rice .The expression analysis of gene under stress helps a lot in studying of stress resistance of rice. A rice gene, OsRAN1, was isolated from rice treated with cold-stress by the fluorescence differential display (FDD) screening method，and its expression pattern was analyzed by simi-quantitative RT-PCR analysis under cold and salt stress. Its cDNA sequence (984bp) contains an ORF encoding a 221 amino acids protein (25.0KD)，and the protein belongs to Ran subfamily of the small G protein, for containing the typical GTPase structure. At the amino-acid sequence level, OsRAN1 exhibit high homology to TaRAN1 and AtRAN1 (98.19% and 92.76%). By simi-quantitative RT-PCR analysis, the expression of OsRAN1 at the transcription level was increased under the low temperature. Especially under high salt condition, the expression level in root shows a process of increase, and then decrease. We suggest the OsRAN1 gene could play an important role of stress response in rice.

Xi Jiang, Du Zhiru, Xu Yongju , Zhang Qi , Wan Jia, Jiang Bin, Xu Zhengjun,. Isolation and Characterization of a Rice Gene OsRan1 Coding a Small G Protein[J]. Chinese Agricultural Science Bulletin, 2008, 24(4): 109-114.

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