Abstract: The experimental boil alcohol extraction isatisroot polysaccharide, and PK-15 cells cultured porcine parvovirus (PPV), by observing cytopathic effect (CPE) to evaluate isatisroot polysaccharide on the porcine parvovirus blocking role and inhibition used. The results showed indigowoad root polysaccharides in the cell concentration of security, porcine parvovirus obviously blocking role and the role of inhibition, the smallest concentration of 2.09μg/ml block, minimum inhibitory concentration for 4.19μg/ml.