Abstract: The effects of drought at seedling stage on anti-ageing enzymes acivities in peanut were studied under water control condition. The results shouwed that daily change of superoxide dismutase (SOD), peroxidase (POD) and catlase (CAT) activity of Luhua 11 was a double peak curve respectively, and reach the first peak at 11:30 and came to the second peak at 15:30. SOD activity of Nongda 818 came to the peak at 13:30, but daily change of POD and CAT activity was a double peak curve respectively, and reached the first peak at 11:30 and the second peak at 15:30. SOD、POD activity of Nongda 818 was much higher than that of Luhua 11. Dought in during seedling stage enhanced anti-ageing enzyme activity in two peanut cultivars, and anti-ageing enzyme activity in Nongda 818 leaves was higher than that in Luhua 11 leaves.