Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2008, Vol. 24 ›› Issue (8): 105-109.

Special Issue: 生物技术 园艺

• 农业生物技术科学 • Previous Articles     Next Articles

Comparison of Protein Extraction Methods for Two-dimensional Electrophoresis in Apple Leaf

Zeng Guangjuan, , Li Chunmin, Zhang Xinzhong, Teng Yunlong, Dong Wenxuan   

  • Received:2008-05-04 Revised:2008-05-26 Online:2008-08-08 Published:2008-08-08

Abstract: In order to develop an efficient protein extraction method suitable for apple proteome analysis, three protocols for total protein extraction in apple leaf, trichloroacetic acid/acetone precipitation (TCA), phenol extraction methanol/ammonium acetate precipitation and Tris-HCl extraction were compared. The extracts were separated by two-dimensional electrophoresis (2-DE) followed by silver staining. The results showed that 450, 374 and 1032 protein spots were detected respectively on 2-DE gels. The Tris-HCl extraction was the most appropriate for apple leaf proteome analysis on account of the highest resolution and more informative 2-DE gels.

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