Abstract: Agrobacterium-mediated method has broad application prospects in the genetic improvement of fruit trees. The Agrobacterium-mediated receptor system has protoplast receptor systems, callus receptor systems, direct bud differentiation receptor system and embryoid Body systems.The factors of affecting the frequency of Agrobacterium-mediated transformation include infection, antibiotics, genotype.Commonly methods of testing include GUS activity detection, PCR technology, hybridization, and immunological detection. This article is reviewed on the Agrobacterium-mediated in a variety receptor system of fruit trees,transforming ways, main factors affecting transfermation of fruit genes and the detections and so on, and provide reference for different fruit trees to establish highly efficient gene transfer systems.