Abstract: In aim to find the SSR markers linked to resistance gene against frogeye leaf spot in Chinese soybean, Bulked segregant analysis was applied to F5 populations derived from cross between the resistant line DongNong40566 and the susceptible line DongNong410, self-crossing for three generations after F2. These SSR markers including Satt565、SOYGPATR and Satt396 were identified from500 SSR markers, the linkage order was Satt565—SOYGPATR—Hrcs1—Satt396, and the distance to the resistance locus was 12.7、6.5、14.7cM respectively. These three markers and the resistance locus were mapped to soybean linkage group C1.