Abstract: [Objective] Studies the best material and the best hormone matching and the best medium on tissue culture and practices seeding and transplant of the Clerodendranthus spicatus.’s in Hainan . Satisfies the industrial production tea of the Clerodendranthus spicatus. to seedling's demand. [Method] Using the stem tips and the stem nodes and the inter-nodes and the new leaves of Clerodendranthus spicatus.in Hainan as explants . Using MS as basic medium, the effects of different concentrations and different types of phytohormone on the germination of stem segment and axillary bud, proliferation of callus and rooting of tufty buds were compared. [Result]The explants disinfection method to be possible to use 70% ethyl alcohol and 0.1% HgCl2 solution minute time interval carries on; The best material is the stem point; The optimum medium for induction was MS+ NAA0.1mg/L +6-BA1.0 mg/L, the optimum medium for subculture proliferation was MS+ NAA0.5mg/L+6-BA1.0mg/L or 2,4-D0.5mg/L+6-BA 1.0mg/L and the optimum medium for rooting of stem segment was 1/2MS+ CM10%+NAA0.1mg/L.[Conclusion] The success has established the Clerodendranthus spicatus in Hainan tissue culture quick numerous technology system, grasped has practiced the related technology which seeding and transplanted.