Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2009, Vol. 25 ›› Issue (12): 195-200.

• 植物保护科学 • Previous Articles     Next Articles

Isolation and Screening of Antagonistic Bacteria against Monilinia fructicola

Hu Haiwen, Yang Haiqing, Wang Peng, Liu Suhua, Zhao Xiaoyan, Liu Zhengping   

  • Received:2009-01-13 Revised:2009-03-26 Online:2009-06-20 Published:2009-06-20

Abstract: Confrontation incubation, 16s rDNA analyse and physiological and biochemical reactions were adopted to screen and identificate the antagonistic bacteria against Monilinia fructicola from peach orchard soil. 11out of 42 bacteria strains isolated from peach orchard soil samples of Pinggu strict, Beijing showed obvious antagonism against Monilinia fructicola. F3 and Y4 had the best antagonistic effects with the antagonistis strip width of 13.83mm and 14.67mm separately. The antagonistic effects of F1 and F4 came second with the anatagonistis strip width of 11.83mm and 12.00mm. F3 and Y4 had teratogenicity to the mycelium morphology of Monilinia fructicola. And the culture filtrate of F3 and Y4 could inhibit the conidial formation and germination of M. fructicola. F1 、F3、F4 and Y4 were identified as Bacillus subtilis.