Abstract: In order to screen the antifungal activity material against Monilinia fructicola from Chinese herbs, three-time-impregnation was used to extract the active material. The antifungal activity was determined by mycelium growth rate, sporulation per unit area and germination rate on slide. The mycelium morphology of M. fructicola was observed by microscope. The results showed that four kinds of solvent extracts of Glycyrrhiza uralensis Fisch，Scutellaria baicalensis Georgi and Scutellaria baicalensis Georgi all can inhibit the growth of M. fructicola in content. Acetone and 80% ethanol extracts of Glycyrrhiza uralensis Fisch inhibited the growth of M. fructicola most with the inhibition rate of 80%. Both the two kinds of extraction can destructd the mycelium morphology of M. fructicola distinctly, inhibit the conidial production and germination, and inhibit the growth of 16 plant pathogenic fungi tested.