Abstract: Phytic acid is the primary storage form of phosphorus（P）in cereal grains. To decrease the phytic acid P content in maize grain is an important approach to prevent the P-pollution. By field trials，the phosphorus content and phytic acid phosphorus accumulation of grain of two varieties of maize under different phosphorus levels were studied. The results indicated that Phosphorus fertilization can increase the total phosphorus content, Phytic acid content and accumulation in the grain. Phosphorus content and phosphorus accumulation of Jin-hai 5 is a little higher than that of Xun-dan20. Phytic acid phosphorus account for 50%-62% of the total phosphorus of maize grain. The proportion of Phytic acid phosphorus to total phosphorus in Jin-hai 5 with high phytic acid is higher than Xun-dan20 with low phytic acid. Increasing the phosphorus levels can reduce the phosphorus transfer rate of root, tassel, rachis and other parts of the two varieties of maize. But the percentage of phosphorus in grain transformed from root, leaves, and ear is not affected by phosphorus fertilization. The relationship of phytic acid P accumulation in grain to phosphorus content and accumulation in ear leaf and bracteata leaf at milky stage, and in seed at ripening period is positive significantly.