Abstract: 【Objective】Plant lectin has important in vivo physiological functions, and it is necessary to expressing BanLec gene in Escherichia coli and investigate its functions. 【Method】Total RNA was extracted from Brazile banana pulp, and the BanLec cDNA sequence was amplified with Reverse Transcription Polymerse Chain Reaction (RT-PCR) and the sequence was analyzed. BanLec was cloned into pET-30a vector, and the recombinant plasmid pET-BanLec was screened. After transforming into Escherichia coli BL21(DE3), the protein was induced to express. 【Result】The Sequence was analyzed with DNAstar software and the result showed that its open reading frame was 426bp and encoded a protein with 142 amino acids. The alignment showed that this gene shared high similarities with other known BanLec genes on the levels of nucleotides and amino acids (94% and 93%, respectively). The result of SDS-PAGE demonstrated that the BanLec gene was expressed by induction and the expression products migrated at a size of 20kD. 【Conclusion】This research is helpful for investigating the activities or other physiological functions of banana lectins.