Abstract: Abstract: Ethylene, an internal source hormone, affects fruit ripening and senescence in plants. ACC Synthase is one of the rate-limiting enzymes of ethylene biosynthesis in plant. The ACC Synthase gene silencing can down-regulate the product of endogeny ethylene in plant. In this study, ACC Synthase gene was cloned with RT-PCR from peach, and then was digested and inserted into the pTRV-RNA2 vector, and transformed into the E. coli component DH5α. The recombination plasmid was selected and identified by restriction enzyme analysis. Sequence analysis showed that nucleotide of the ACC Synthase gene was 99% identical to the published sequence. The results showed that ACC Synthase gene was rightly cloned into the gene silencing vector pTRV-RNA2.