Abstract: Pot plant experiment was conducted to study the effect of the different fertilizer made from animal tissues on content change and relationships of micro-elements nutrient in rhizosphere soils and leaves of two-year Xiangling walnut (Juglandaceae nigira cv.xingling). Six kinds of formulations of blood meal, egg shell meal, chicken manure and ammonium phosphates were selected as the fertilizer treatments to measure the content and relationships of Fe, Mn, Cu, Zn in rhizosphere soils and leaves of young walnut in growth period. The result showed that: the content of the micro-element in rhizosphere soils of walnut showed the tendency to increase first and then decrease and the change trend of the content of the micro-element in leaves was different with fertilizer treatments. Compared with the control, the content of Fe, Mn, Cu, Zn in rhizosphere soils and leaves of walnut were improved significantly, but the promoting effects of different fertilizer treatments on elements were different on test point. Correlative analysis showed that there were significant positive relationships among the micro-elements in rhizosphere soils and leaves of walnut, there were some significant negative relationships among the micro-elements in the content of elements of rhizosphere soils to that of leaves of walnut plant. It was speculated that comprehensive effect of the formulation of fertilizer made from animal tissues--blood meal:40g,egg shell meal:30g,ammonium phosphates:10g（T6）on Fe, Mn, Cu, Zn in rhizosphere soils and leaves of walnut was better than other treatments.