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Chinese Agricultural Science Bulletin ›› 2009, Vol. 25 ›› Issue (8): 62-64.

Special Issue: 生物技术

• 农业生物技术科学 • Previous Articles     Next Articles

Comparing Study on several Methods for DNA Extraction from endophytic fungi

Wu Fahong, Huang Dongyi, Huang Xiaolong, Zhou Xin, Cheng Wenjie   

  • Received:2009-02-18 Revised:2009-03-03 Online:2009-04-20 Published:2009-04-20

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Abstract: In this study, three different methods were used to extract DNA from endophytic fungi: CTAB,SDS and the improved CTAB. Quality and quantity of the extracted DNA were compared for the there methods. The results showed that all of the there methods could obtain the DNA, but the improved CTAB could obtain the highest quantity of the purest fungal DNA. The purest DNA were amplified by PCR .The clear and singal gel fragment showed that the extracted DNA could usde for the other biological experiments.