Abstract: With the purpose of extending storage period of sweet sorghum stalks, the content changes of total sugar, soluble sugar, reducing sugar, sucrose and cellulose inside stalks of New No.4 Sweet Sorghum were studied under the conditions of both vertical stacking storage and horizontal stacking storage at 157-199-day storage period. The results showed that the content of total sugar, soluble sugar, reducing sugar, sucrose inside stalks of horizontal stacking storage are higher than that of vertical stacking storage . Cellulose content is almost same with both storage methods. This research provides a scientific reference for solving storage problems of stalks during fuel ethanol production with sweet sorghum stalks.