Abstract: Cymbidium faberi ’cui mei’ and common Cymbidium faberi were employed as parents. The hybrid seeds were germinated in axenic culture in different budding phase and different agar medium to contradistinguish.We also study the propagation and differentiation of PLB of crossbreed. Several different mediums were used to study the propagation of PLB of crossbreed. Three facts of medium,6-BA and NAA were designed to study the effects on differentiation of rhizome.The results show that the best bud medium was KC and the best reap time is 110d after the pollination.The best propagation medium was W+NAA0.5mg/L+BA1.0mg/L, The best plantlet regeneration medium was MS+NAA 1.0 mg/L +6-BA 0.5 mg/L.