Abstract: Light is one of the most important factors in the growth of plant，and the virtues of LED ，such as small volume and mass,longer life，wavelength specificity etc.,make it possible for it to be used as an important instrument in the regulation and control of plant light environment.And it indicates that LED has an significant advantage in energy conservation and the promotion of plant growth.At present,the LED has been applied abroad in the researchment of plant tissue culture,and it has gets some valuable results.This article introduces the main characteristics of LED and the appliaction of LED in plant tissue culture.