Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2009, Vol. 25 ›› Issue (13): 37-39.

Special Issue: 园艺

• 农业生物技术科学 • Previous Articles     Next Articles

Analysis of SSR Information in EST Resources of Kiwifruit (Actinidia ssp.)

Jiang Chunya, Xu Xiaobiao, Liao Jiao, Ni Zhihua, Li Jing   

  • Received:2009-03-27 Revised:2009-05-07 Online:2009-07-05 Published:2009-07-05

Abstract: 56,400 EST sequences were randomly selected from EST(Expressed Sequence Tag)sequences of kiwifruit in the database of NCBI. EST sequences were analyzed and the SSR (Microsatellite)could be found out by using the SSRHunter software. The results showed that the 7939 SSRs were identified from the randomly selected kiwifruit EST resources. Among which there were 5131(64.63%)dinucleotide,1237(15.58%)trinucleotide, 284(3.58%)tetra nucleotide, 397(5.00%)pentanucleotide and 890(11.21%) hexanucleotide SSRs. The frequency of SSRs was approximately 1/2.48 kb, which could exist to 1 SSR in 7 unigenes. The dinucleotide repeats appeared to be the most abundant SSRs, followed by trinucleotide and hexanucleotide repeats. Among them the repeat motif such as AG/CT was predominant in each type of SSRs accounting for 4654(90.70%). We screened the SSR repeats in the EST of kiwifruit (Actinidia spp.), and will make better use of the EST-SSR resources of kiwifruit for further molecular biological studies.