Abstract: Abstract: Compared to Camptotheca acuminata Decne come from Sichuan (SC),the content of camptothecin (CPT) in different canopy of plant C. acuminata ‘Hisun-I’(HI) branch leaves in the whole growing season of three years have been detected dynamically by HPLC. The results showed that the content of CPT in HI was higher than SC. According to the demand of downstream extraction technology, the harvest seasons of HI should be june or september. The branch leaves of HI was suitable for consecutive years harvesting，and the harvest cutting cycle shuould be between eight and ten years.