Abstract:

Abstract：【OBJECTIVE】Sequence-related amplified Polymorphism(SRAP) is a fairly new PCR-based molecular marker technique developed recent years. The objective of this study was to discuss the possibility of applying SRAP in maize hybrid identification, and to develop identification system. 【METHOD】Nineteen maize hybrids were analyzed by SRAP technique with 25 pairs of SRAP primers. 【RESULTS】Nineteen pairs out of 25 pairs of SRAP primers were selected, which displayed polymorphism, stable and repeated amplification profiles. A total of 152 polymorphic SRAP bands were detected and one primer pair produced 8 polymorphic bands on average. The ratio of polymorphic bands was from 44.4%（me4/em3）to 91.7%(me4/em1). The Polymorphic Information Content (PIC) for the SRAP varied from 0.752 to 0.922 with an average of 0.879. Prominently, primer pair me4/em1 can distinguish the 19 maize hybrids by itself. Furthermore, 5 primer pairs were selected and fingerprinting of 19 maize hybrids were developed, which would be basement of maize identification by SRAP technique. 【CONCLUSION】The results verified very high discrimination ability of SRAP markers, moreover, considering its simple, stable, co-dominant and well- proportioned distribution, conclusion could be made that SRAP might be a potentially useful marker technique and supplement for SSR in maize hybrid identification.