Ca2+ involved in Nitric Oxide content of wheat seedling leaves induced by SNP under drought stress

Abstract

Abstract: Under 15% PEG-6000 Drought stress condition, the effects of sodium nitropprusside (SNP, an exogenous nitric oxide donor) at the concentrations of 1mmol/L SNP on NO content, Nitric Oxide-synthesis enzyme activities and the relation with Ca2+ in wheat seedlings leaves were investigated under drought stress. The result is that 15% PEG could apparently enhance NOS activity in wheat seedling, NO was quickly produced by cNOS, and the proportion of iNOS in NOS was increased slowly after 6h treatments. NO was also induced by NR, which was a little part of total NR activity. The 1mmol/L SNP treatment could apparently alleviate the drought-induced membrane lipid peroxidation, which consistent with the up-regulation of NOS, NR and NO. The effect of SNP was decreased after treatment of blocker of plasma membrane Ca2+ channels LaCl3. The above results indicated that exogenous nitric oxide donor (SNP) could apparently increase Nitric Oxide-synthesis enzyme activities and NO content, and Ca2+ was involved in regulation of NO level in wheat seedling leaves by SNP under drought stress.

References

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