Involvement of Ca2+ in ethylene biosynthesis of Cut Rose During Vasing regulated by Nitric Oxide

Abstract

Abstract: Cut rose ‘Kardinal’ were treated with 0.1 mmol?L-1 SNP(nitric oxide doner), 0.1 mmol?L-1 SNP plus 0.3 mmol?L-1的TFP(CaM inhibitor),0.1 mmol?L-1 SNP plus 10 mmol?L-1的TFP(calcium chelator), 6 mmol?L-1 Ca2+, 6 mmol?L-1 Ca2+ plus 0.05 mmol?L-1的PTIO(nitric oxide scavenger ) for 3 h. The objective of this study was to understand the effect of Ca2+ in ethylene biosynthesis of cut rose during vasing regulated by nitric oxide (NO). The result showed that Exogenous Ca2+ treatment improved NOS activity during the former vasing, maintained relative higher NO content in petals, postponed the increase of NOS activity during the latter vasing. Further study showed that EGTA and TFP treatment stimulated ACS and ACO activity, accelerated the increase of ACC content, advanced the ethylene biosynthesis in petals during vase; At same times, PTIO also inhibited the decrease of ACS, ACO activities and ACC content which induced by Ca2+ treatment. This also proved that Ca2+ and CaM participated in regulation of endogenous ethylene biosynthesis and signal transduction during cut rose vasing.

ZHANG Shao-Ying ,. Involvement of Ca2+ in ethylene biosynthesis of Cut Rose During Vasing regulated by Nitric Oxide[J]. Chinese Agricultural Science Bulletin, 2009, 25(13): 82-87.

References

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