Abstract:

The total DNA was isolated from the leaf of Taihanggia rupestris YU et LI by the conventional CTAB method，improved CTAB method and SDS method. The extracts by improved CTAB method were tested as the template for several molecular marker types．The results showed the extracts by conventional CTAB method were brown and could not dissolve completely, and those by SDS method had a low quality and yield. As for the improved CTAB method, there was a high production rate of the extracts，that was pure with little degradation，and A260/ A280 was 1.8 or so. With these leaf DNA serving as template, PCR at two loci of mitochondrial and chloroplast genomes was effective and special highly. Besides, these total DNA were good templates for SSR and RAPD molecular markers. So improved CTAB method is a good protocol by which the total DNA was isolated from the leaf of T. rupestris, its product meet the requirement of PCR amplification for the nuclear, chloroplast and mitochondrial genomes.