Abstract: Reyan88-13, a superior clone of rubber tree, was used as material to study the affects of SAM-SSR (digestion, linkage, suppressed amplification and pre-amplification PCR). The results showed that the optimal digest concentration, method and time of enzyme were 5U MseⅠand 5U PstⅠ,double digest for 1h; the optimal concentration of DNA template was 100~300 ng/ul; the optimal temperature and time of linkage were 16℃ overnight; And the suppressed amplification and pre-amplification PCR were set up based the productions of linkage and suppressed amplification diluted by 20 times respectively. The productions of SAM amplification were detected by 8% Polyacrylamide Gel Electrophoresis and the results showed, the amplified productions ranged from 200bp to 750bp and could be used to construct the SSR genomic library for rubber tree.