Abstract:

In March 2008,a piggery in Xinxiang city where broke out a disease characterized by sows abortion ,stillbirth, produceed the mummy foetus, dead foetal and weak piglet.Colleted the aborted fetus and the dead foetal lung , liver and spleen ,useed these diease samples to make suspension ,to obtain supematant,and to have an inocalation toxin to carry out cell culture,then observed the pathological changes cell.According to porcine reproductive and respiratory syndrome virus Americas strain VR-2332 gene sequence, designed a pair of specific primers, extracted PRRSV RNA from abortion fetal lung、liver and other tissues, the result was positived by RT-PCR amplification, in light of history and clinical symptoms, checked it out to be porcine reproductive and respiratory syndrome. The piggery took a comprehensive control measures and achieved good results. The farm took some emergency vaccination ,disinfection and other comprehensive prevention measures to control the epidemic and the spread of the disease.