Abstract: Abstract：Streptomyces roseoflavus Men-myco-93-63 was isolated from potato scab (S. scabies) decline soil and its fermentation can inhibit many phytopathogenic fungi and control related important plant diseases such as cotton verticillium wilt (Verticillium dahliae) and cumber powdery mildew (Sphaerotheca fuliginea Poll). The genetic integrate plasmid pSET152 and genetic damage plasmid pKC1139 used as the starting plasmid, ET12567 (PUZ8002, pSET152) and ET12567 (PUZ8002, pKC1139) used as the donor, Men-myco-93-63 spores and hyphae used as the receptor, respectively, the system for the conjugal transfer of pSET152 or pKC1139 from Escherichia coli in to Men-myco-93-63 has been developed. Different medium, such as MS, PDA, TSB agar, oats medium were selected for conjugal transfer, respectively. The results showed that MS medium is fit for conjugal transfer between Men-myco-93-63 and ET12567 (PUZ8002 ), and it was confirmed that plasmid pSET152 and pKC1139 had been successfully transferred to the Men-myco-93-63, respectively. When spores were used as the receptor, the pre-conditions of the germination of spores were 50 ℃ heat shock 10 min, 37 ℃ incubated 2.5 h in MS medium, and the conversion efficiency was 10-7 to 10-6. If the mycelium were uesd as the receptor, the conversion efficiency was 10-7 to 10-6, but the mycelium pollution can not be avoided. In addition, the coverage time on antibiotics effected the joint transfer efficiency significantly, 16 to18 hours was best.