Abstract: Abstract: Physiological index of leaves were analyzed and frozen injury in field were investigated, which were sampled from thirty specimens of tea germplasm resources proved to have a high index of leave anatomical structure. The results indicated that four indexes of those gemplasms varied significantly, including relative permeability of plasma (X1), content of soluble sugar (X2), content of soluble protein (X3) and frozen injury. The computing formula of physiological index of leaves (Y1) was made to be Y1 = 0.45X1 + 0.3X2 + 0.25X3. The stepwise regressive model of frozen injury index (Y2) was made to be Y2 = 2.799 + 0.050X1 - 0.401X2 - 1.850X3. Those germplasms could be clustered to three different groups, among which nineteen germplasms belonged to the first group and the second group were well-judged to have strong resistance to cold. The evaluation system built by physiological index of leaves could be used to identify cold resistance in period of young seedling, which could remarkably raise the efficiency in screening tea germplasm resources resistant to cold.