Abstract:

Abstract：【OBJECTIVE】the research aimed to study the tube propagation process of induction, differentiation, rooting,transplanting of Glehnia litteralis,and to screen fine tube seedlings.【METHODS】the rhizome of Glehnia litteralis was used for explant,MS for basic medium,adding different hormone mixed at different ratio, under different conditions of light intensity and other measure.Then the research of adventitious buds inducement, rooting, acclimatization and transplants was carried out to find the best cultivation.【RESULTS】the suitable medium for adventitious buds inducement from rhizome in vitro is MS medium+6-BA2.2~2.4 mg?L-1+NAA 0.2mg?L-1+Sucrose30mg?L-1＋Agar8mg?L-1. The suitable medium on rooting of adventitious buds in vitro is 1/2MS+IBA 0.2 mg?L-1+Sucrose 15mg?L-1＋Agar 6mg?L-1.The survival rate of adventitious buds cutted with stem segments was significantly enhanced. 1/2MS medium with 0.02%active carbon could increase average roots per plantlet and could improve root hair’s development. Semienclosed-type acclimatization that MS medium covered about 2 days and then in mixed medium about 10 days is effectly helpful to improve plantlets’ survival rate. The suitable two mixed mediums for transplant are 1/3peat+ 1/3 sand+1/3garden soil and 1/3vermiculite+1/3peat+1/3garden soil.