Abstract: In this paper Cattleya hybrida was used as the main test material to study the technology of hydroponic culture. With different concentrations of 1-naphthlcetic acid (NAA) treatment to induce aquatic root system, the aim was to choose the best concentration of NAA. After the water-cultivated-root growed, different nutrient solutions (clear water, 1/4MS, Hoagland solution and the KC nutrition) were used to culture the plantlet of Cattleya hybrida in order to find suitable nutrient solutions to promote Cattleya hybrida growth and development. During the culture of Cattleya hybrida, the other aim was how to prevent root rot of Cattleya hybrida. The results showed that lower concentrations NAA could promote water-cultivated-root to grow, the best concentration of NAA was 5mg/L for the induction of root. The reasons causing root rot were that plants could not inhale enough oxygen, the NAA concentration was too higher, or the algae growth. Not using Pharmacy, reducing the concentration of NAA, or changing water usually would prevent root rot. KC nutrition was a better nutrition for Cattleya hybrida.